Evaluation of free-radical quenching properties of standard Ayurvedic formulation Vayasthapana Rasayana

Citation: BMC Complementary and Alternative Medicine 2011, 11:38



Cellular damage induced by free-radicals like Reactive Oxygen and Nitrogen Species (ROS and RNS) has been implicated in several disorders and diseases, including ageing. Hence naturally occurring anti-oxidant rich-herbs play a vital role in combating these conditions. The present study was carried out to investigate the in vitro free-radical quenching capacity of a known Ayurvedic poly-herbal formulation called Vayasthapana Rasayana.


Methanol extracts of Vayasthapana Rasayana formulation (VRF) were studied for in vitro total antioxidant activity along with phenolic content and reducing power. In vitro assays like DPPH, FRAP, ABTS scavenging to evaluate radical quenching potential were performed.


The formulation has shown 94% at 0.1 mg/ml DPPH free-radical scavenging activity as against 84% at 0.1 mg/ml for standard ascorbic acid (IC50 value 5.51 μg/ml for VRF and 39 μg/ml for standard). It has a significant higher ferric reducing potential also (OD 0.87 at 700 nm & 0.21 at 0.1 mg/ml for VRF and standard, respectively). The total phenolic content (gallic acid equivalent) of the VRF is 8.3 mg per g of dry mass. Total antioxidant capacity of the formulation, estimated by FRAP was 1150 ± 5 μM Fe(II)/g dry mass. ABTS radical scavenging activity of VRF was 69.55 ± 0.21% at 100 μg/ml concentration with a IC50 value of 69.87 μg/ml as against 9% and 95% by ascorbic acid and Trolox (at 70.452 μg/ml and 0.250 μg/ml concentrations, respectively).


In Indian traditional Ayurvedic system, use of VRF is in regular practice for mainly combating age-related disorders and diseases as many of the components of the Rasayana are known for their free-radical scavenging activity. This study has validated the potential use of VRF as an anti-oxidant to fight age-related problems.

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Dr. Omkar Kulkarni

Ayurveda Specialist


2 thoughts on “Evaluation of free-radical quenching properties of standard Ayurvedic formulation Vayasthapana Rasayana”

  1. Dear Doctor, Thanks for sharing this research. The search for anti-ageing molecules is something that globally has attracted interest. “Life extension” is a very interesting magazine that is released by researchers in US and a lot of our doctors actively refer to. Pls notice here how curcumin has been researched extensively in US for same : http://genomics.senescence.info/drugs/visualisation.php

    A lot of sponsors are interested in looking and investing in anti ageing drugs. For human tests we are exploring cellular ageing/ chromosome ageing through telomere testing in humans? would you be in agreement for the same or you think other biomarkers might be more suitable?

  2. I believe this is quite valuable to search for these markers. We had also tried to see the effect on SIR2 genes but the work did not reach completion due to some administrative issues.
    Thanks and would like to receive update on your research. Good luck.

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