A Study Of Changes In RBC Indices & Electrolytes After Blood Donation

*Dr. Angad B. Tanibade, Author
**Dr. Vaibhav.-B. Wavhal, Co-Author


To rule out the various misconception about Blood donation. a study was undertaken bye valuating the effect of Blood Donation on the RBC indices, Hb% & Sr electrolytes In this study 300 ml blood of healthy individual was letted out and RBC Indices Assessed on Day 1st ,7th’,14th 2 lst &30th It was found that there is no effect of B.D. on RBC indict i.e. RBC Count MCV, MCH, MCHC Hb% & electrolytes In All followup it means Physiological parameters mentioned above remains at normal level. Thus Blood Donation doesn’t harms or. do sent produce any Adverse effects over Body.

Key words

RBC indices i.e. RBC Count, MCV, MCH & MCHC has important role in blood physiology.



For the Healthy life,’the body constituents should be maintained in homeostatic condition and this is gained by adequate blood proportion in Human body.

According to the ancient Acharya, Dhatu’s are explained in their reputed documentations i.e. Rasa- Rakta- Mansa-Meda- Asthi, Majja & Shukra. Amongst the 7 Dhatu’s, Rakta. i.e. Blood is given the supreme position by Acharya Susruta as the nutrition of all” the other elements depends on blood The Main function of blood is to provide life i.e. Jivan (life) to living beings. Any fluctuations i.e. reduction or increase in its quantity/quality may lead to various pathology.


Misconceptions Are as follows

“I Might Catch A Disease.”

A new, sterile needle is used for each donor and then immediately disposed it properly. It is not possible to catch AIDS or any infectious diseases by donating blood.

“It Might Hurt When They Draw The Blood.”

The actual drawing causes no pain. You might feel a slight pinch when the needle is inserted, but that’s all.

“I’m On Medication.”

Many medications including allergy medicines, vitamins,aspirins, birth control and blood pressure pills, do not affect your eligibility as a blood donor. Be sure to tell the medical interviewer what medications you are taking or have recently taken.

“I’m Anaemic.”

A trained technician will test a drop of your blood before you donate. If you are anaemic you will not be permitted to donate that day. However, anaemic is not usually a permanent condition. Just because you have been deferred or told that you were anaemic does not necessarily mean that you cannot give blood later.

“They’ll take Too Much Blood And I’ll Feel Weak”

If you weight at least 110 Ibs, you may safely donate blood. Only one pint is taken You can about two “pints of blood for every 25 pounds of body weight and your body makes new blood constantly. After giving blood.most people can resume their normal activities.

“My Blood Type Is So Common, You Probably Don’t Need It.”

Not true. Some blood types are more common than others, but the rarest type of blood is the type that is not available when needed. Since whole blood and blood components must be transfused within a few weeks, we rely on regular donations of all blood types through out the year.

“Other People Must Be Giving Enough Blood.”

We need to collect about 450 pints of blood per day to meet the daily demand and the demand is rising! But,less than 5 percent of those who are eligible give . blood.Consequently, the lives of many depend on the generosity of these few.

“I Give My Blood Voluntarily, But The Blood Center Turns Around And Sells It.”

BCP is a nonprofit organization. The blood center’s goal is to keep costs as-low as possible while maintaining a high quality blood supply. BCP charges a processing fee to recover the costs incurred in collecting, processing,testing and distributing the blood. This processing fee does not include hospital charges for cross matching,transfusion administration etc.

“I’m Too Busy.”

Is your life worth an hour of someone’s time? Is someone else’s life worth an hour of your time? Think about it. on completion this study shows no changes in all parameters i.e. RBC Indices, Hb % & sodium potassium in all follow-ups.


60 healthy individuals having Hb more than 11 gm % were selected from Government Ayurved College-Hospital, &it’s periphery for this study.



1) Individuals of 18 to 50″ yrs of age were selected for study.

2) Male &’Female individuals were considered irrespective of the religion, caste & region.

3) Healthy individuals having Hb% more than llgm %were considered for the study.

4) Fully co-operative and convinced individuals were selected.



1) Individuals Age below 18 years and above 50 years were not consider for research work.

2) Individuals. having any major illness and blood disorders were excluded for research work.

3) Individuals on Anticoagulant drugs-were not considered.

4) Immuno compromised (eg. HIV individuals) were excluded from the Research study.



1) The research work were carried out during the period Oct-Nov 2010

2) After complete Examination And necessary investigations healthy individuals were selected for Blood Donation.

3) Tourniquet was used for pressure.

4) 18 No. Scalp Vein set was used for puncturing the vein.

5) Sterile blood preserving bags were used for collecting blood.

6) Sterilized cotton pad and bandage were applied for pressure bandaging after Blood Donation .

7) Blood Donation was done under all aseptic precautions.

8) Consent was taken to evaluate blood investigation and Blood Donation procedure from individuals.

9) Proforma of individuals were filled.

10) Selection of individuals for study were done, after all the Routine Investigation.



1) Individual was taken in lying position on the bed.

2) Cubital vein was selected for the Blood Collection

3) Tourniquet was applied 6 cm away from site of collection towards heart.

4) Under all aseptic precautions vein was punctured by16 no. needle and allowed for blood collection 300ml. in sterile blood preserving bags & then collected blood was send to Blood Bank.

5) Tourniquet was removed.

6) Needle was removed & tight pressure bandage was applied.

7) Individual was advice to take energetic Fruit And Biscuts orally after procedure.

Blood sample were collected one. day, before of Blood Donation and again on 1st, 7th, 14th, 21st & 30th day after- Blood Donation procedure and’ were studied for Total RBC Count, Hb% , RBC indices, Serum Na and K.



Haematological criteria




Parametric Data




Effect of Blood Donation with reference to parameters:

The changes observed during the clinical study were note dafter every follow-ups and represents by statistic method. For multiple comparison one -wayAnova test wasapplied & results were as follows.

Effect on Hb%: No significant changes were noted in Hb% in all follow ups.

Effects on R.B.C.: No changes were noted in all follow-ups in RBC count.

Effects on MCV: No significant changes were noted in all follow-ups in MCV values.

Effects on MCH :No changes were noted in all follow upsin MCH value.

Effects on MCHC: No significant changes were note din MCHC value in all follow ups.

Effects on Na: No significant changes were noted in Na in all follow-ups.

Effects on K : No significant changes were noted in K value.



As Blood Donation is a Very important Donation, Govt.Recognize it as national work. but there are unevidenced misconception that Blood Donation affects blood constituents like RBC Indices, Hb%, & Electrolytes etc.This study overcome misconception i.e.it Cause anemia & It Harms body by Affecting Blood Constitution, but by evidencing the insignificant results on hematological parameters and unfounded any Adverse effect in studied individuals hence Blood Donation can be perform safely.


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Moderator Shashikant

Ayurveda Specialist


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